Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress

Female, especially for pregnant female, are vulnerable to psychological stress. The morphology and metabolism of the maternal intestine both obviously changed during pregnancy, thus making intestinal health status more fragile under aim present study was investigate role CRH CRHR1 in stress, exploring mechanism stress intestine. Bama miniature pigs were divided into control restraint groups from first day pregnancy. After treatment 18 consecutive days (D18), plasma, duodenum, jejunum, ileum colon collected study. Pregnant subjected had significantly elevated CRH, adrenocorticotropic hormone (ACTH) cortisol (COR) levels plasma. Consistent with increase levels, we observed enhanced oxidative intestine, which resulted mucosal injury, including impaired morphology, a reduced number goblet cells proliferating cell nuclear antigen‐positive cells, decreased expression MUC2 tight junctions, caspase-3. Moreover, exogenous could directly promote IPEC-J2 apoptosis influence its cycle (S G2 phase) through CRHR1, antalarmin alleviate this phenomenon. Therefore, our results illustrated that dysfunction caused by these changes associated